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Enzymological Characteristics of Avian Influenza A Virus Neuraminidase

Identifieur interne : 002329 ( Main/Exploration ); précédent : 002328; suivant : 002330

Enzymological Characteristics of Avian Influenza A Virus Neuraminidase

Auteurs : Katsuhiro Kiyotani ; Naomi Takei ; Masato Senoo ; Shinichi Takao ; Koichi Otsuki ; Misao Tsubokura ; Tetsuya Yoshida

Source :

RBID : ISTEX:A62E6748B3A00DB371E771E6136DEE96BB70CAEF

Abstract

Neuraminidases of 18 strains of avian influenza A virus were examined by both colorimetric and fluorometric assays using fetuin and 4‐methylumbelliferyl‐N‐Ac‐α‐d‐neuraminide as substrates, respectively, to compare them with those of human influenza A and B viruses. The ratios of the neuraminidase activity of avian influenza virus measured by the colorimetric assay method to that measured by the fluorometric assay were distributed in the range of 2.4–20.3. The enzyme of avian influenza virus showed calcium‐ion dependence in both assay methods. These results suggest that neuraminidase of avian influenza A virus is varies greatly from one strain to another in substrate specificity as compared with those of human influenza A and B viruses, and that some strains of avian influenza A virus have a neuraminidase with unique enzymological characteristics different from that of human influenza A virus as well as that of influenza B virus.

Url:
DOI: 10.1111/j.1348-0421.1987.tb01347.x


Affiliations:


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<div type="abstract">Neuraminidases of 18 strains of avian influenza A virus were examined by both colorimetric and fluorometric assays using fetuin and 4‐methylumbelliferyl‐N‐Ac‐α‐d‐neuraminide as substrates, respectively, to compare them with those of human influenza A and B viruses. The ratios of the neuraminidase activity of avian influenza virus measured by the colorimetric assay method to that measured by the fluorometric assay were distributed in the range of 2.4–20.3. The enzyme of avian influenza virus showed calcium‐ion dependence in both assay methods. These results suggest that neuraminidase of avian influenza A virus is varies greatly from one strain to another in substrate specificity as compared with those of human influenza A and B viruses, and that some strains of avian influenza A virus have a neuraminidase with unique enzymological characteristics different from that of human influenza A virus as well as that of influenza B virus.</div>
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